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nextseq 500 550 high output v2 5 kit  (Illumina Inc)


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    Structured Review

    Illumina Inc nextseq 500 550 high output v2 5 kit
    Nextseq 500 550 High Output V2 5 Kit, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 98/100, based on 2230 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/nextseq+500/NextSeq+500%2F550+High+Output+Kit+v2%2E5/pm41933934-331-11-19
    Average 98 stars, based on 2230 article reviews
    nextseq 500 550 high output v2 5 kit - by Bioz Stars, 2026-09
    98/100 stars

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    Related Articles

    other:

    Article Title: Intestinal B cells license metabolic T-cell activation in NASH microbiota/antigen-independently and contribute to fibrosis by IgA-FcR signalling
    Article Snippet: The library was sequenced on a NextSeq 500 (Illumina) with 63 cycles for the cDNA in read1 and 16 cycles for the barcodes and UMIs in read2.

    Article Title: Intestinal B cells license metabolic T-cell activation and contribute to fibrosis via IgA-FcR signalling in NASH
    Article Snippet: The library was sequenced on a NextSeq 500 (Illumina) with 63 cycles for the cDNA in read1 and 16 cycles for the barcodes and UMIs in read2.

    Article Title: Exosome-Derived miR-11987 in Bovine Milk Inhibits Obesity Through Browning of White Fat.
    Article Snippet: The library was sequenced on NextSeq 500 (Illumina, Inc., San Diego, CA, USA) with single-end 75 bp reads.

    Sequencing:

    Article Title: 18DS for 48hrs at the indicated concentrations before flow cytometric analysis for Annexin V and propidium iodide (PI) staining. (B) OPM2 cells were treated with 18DS for 48hrs prior to fixation and staining of DNA for SubG1 fraction by PI. (C) OPM2 parental cells and OPM2 MSCV-BCL2 cells were treated with 18DS for 48hrs and viability assessed by TMRE
    Article Snippet: ChIP DNA quality was assessed using the Aligent 2100 Bioanalyser (Agilent Technologies) and measured using the Qubit dsDNA HS Assay Kit (Thermo Fisher Scientific). .. The KAPA Hyper Prep Kit was used to generate libraries which were sequenced on the NextSeq 500 (Illumina). bcl2fastq (Illumina) was used to demultiplex raw sequencing reads and generate FASTQ files which were mapped to the reference genome using Bowtie (v2.2.3). ..

    Article Title: Tracking breast cancer progression using Methylscape
    Article Snippet: For DNA fragmentation, a Covaris S2 sonicator was used (Covaris), and library preparation for both WGS and WGBS was carried out using the NEBNext Ultra II DNA Library Prep Kit and NEB E7760 or E7595s RNA library kits (New England BioLabs). .. Sequencing was performed using Illumina platforms: the NextSeq 500 and NovaSeq 6000 systems. .. Quality control and size distribution analysis were conducted using the Agilent Bioanalyzer (Agilent).

    Article Title: Correlated protein-RNA associations reveal a requirement for HNRNPU in long-range Polycomb recruitment by the lncRNAs Airn , Kcnq1ot1 , and Xist
    Article Snippet: All DNA size-selection purification steps were performed using AMPure XP beads (Beckman Coulter, cat #: A63880). .. Single-end, 75-and 100-bp sequencing was performed using Illumina NextSeq 500 and 1000 systems, respectively. .. irMEF feeder-depleted TSCs were washed twice with ice-cold PBS and harvested with 1 mL TRIzol (Thermo Fisher 15596026).

    Suspension:

    Article Title:
    Article Snippet: .. After the target cell suspension was examined for quality and cells were counted using a hemocytometer, they were delivered into a Chromium Controller (10X Genomics), captured and libraried with the Chromium single cell 3’ reagent kit v3 (10X Genomics). cDNA libraries were sequenced on NextSeq 500 (Illumina) using the High Output 75 Cycle kits to a minimum depth of 65,000 mean reads per cell. .. Reads were demultiplexed and aligned to the mm10 reference genome using the Cellranger pipeline (v 5.0.0, 10X Genomics).

    Single Cell:

    Article Title:
    Article Snippet: .. After the target cell suspension was examined for quality and cells were counted using a hemocytometer, they were delivered into a Chromium Controller (10X Genomics), captured and libraried with the Chromium single cell 3’ reagent kit v3 (10X Genomics). cDNA libraries were sequenced on NextSeq 500 (Illumina) using the High Output 75 Cycle kits to a minimum depth of 65,000 mean reads per cell. .. Reads were demultiplexed and aligned to the mm10 reference genome using the Cellranger pipeline (v 5.0.0, 10X Genomics).

    cDNA Library Assay:

    Article Title: Hepatocyte mARC1 promotes fatty liver disease
    Article Snippet: Transcriptome (mRNA) Library Preparation and Sequencing for in Vivo Samples 11 10 ng-1 μg purified RNA from each sample was used to generate a cDNA library using the NEBNext® UltraTM II Directional RNA Library Prep Kit for Illumina. .. The cDNA library was then sequenced on a NextSeq 500 using NextSeq 500/550 High Output Kit V2 (Illumina). .. Sequence reads were again aligned using STAR (18) against the Ensembl mm10 transcriptome annotation.



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